SRY testing for female athletes

Absence or presence of the SRY gene as an indication for biological sex

SRY testing for female athletes

A reliable method for verifying genetic sex, especially in contexts where accurate sex classification is required, such as elite sports or certain medical evaluations. It is a fast, non-invasive, and scientifically validated tool that supports fairness, regulatory compliance, and informed clinical decision-making.

To promote fairness in women’s sports, the World Athletics Federation has implemented a policy requiring genetic testing for the presence or absence of the SRY gene as an indicator of biological sex. Beginning September 1, 2025, all athletes intending to compete in the female category at the World Championships must undergo a one-time SRY gene test.

Amplexa Genetics has developed a molecular test for the SRY gene. The test is intended for elite athletes wishing to compete in national and international competitions. The test is based on a quantitative Polymerase Chain Reaction (qPCR) of the SRY gene (Sex determining Region Y), a test which has a very high specificity and sensitivity.

The Analysis

The report will be giving the status on the absence or presence of the SRY gene as an indication for biological sex and the result is solely intended for classification into a sporting category.

In case a female athlete shows the presence of the SRY gene we recommend clinical genetic counselling. Amplexa Genetics can deliver clinical genetic counselling and molecular genetic follow up analyses for the presence of a Y-chromosome or sex chromosome ploidy. We also have collaboration with hospital who can deliver the biochemical follow up analyses and clinical assessment for EG insensitivity syndrome.

Amplexa Genetics can deliver the full service – from sampling, molecular genetic analysis, genetic counselling and via our collaborators we can deliver follow up clinical analysis in case of positive testing.

Test Specifications

  • The test is conducted using a standard qPCR based probe for the SRY gene. As internal control for every sample reaction human housekeeping gene is used as validation for the functionality of the reaction mix and the added DNA.
  • Test results are provided within the same day of sample processing.
  • The test is performed in an ISO15189 accredited environment.

Report And Documentation

Analysis reports are delivered anonymously directly to the athlete through a secure e-mail system. The report is delivered as a pdf document with a digital document verification system. The digital document verification system ensures that the results can’t be modified in the document.