TP-PCR

Uncovers repetitive sequences with triplet repeat-primed PCR

Triplet Repeat-Primed PCR (TP-PCR)

Our cutting-edge service harnesses TP-PCR, a highly sensitive technique tailored for the detection of repetitive DNA sequences.
More Information

TP-PCR employs specially designed primers that target triplet repeat motifs, allowing for precise amplification and detection of these sequences with unparalleled accuracy.

Unlike conventional PCR methods, TP-PCR excels in identifying expansions or contractions of repeat sequences associated with various genetic disorders, offering invaluable insights into disease mechanisms and diagnostics.

Empower your research with our customizable TP-PCR solutions, delivering precise and efficient detection tailored to your unique experimental needs.

Advantages using TP-PCR

  • Increased clinical accuracy compared to Southern Blot
  • Low abundance total mutations are detected
  • Increased resolution on female zygosity
  • Repeats of AGG interruptions are detected
  • Diagnostic Sensitivity of 100%; Diagnostic Specificity of 98.4% and overall accuracy of 99%

Test Specifications

Chemistry: CE IVD approved AmplideX® PCR/CE from Asuragen Hardware: SeqStudio Genetic Analyzer

Data processing: Data is analyzed inGeneMarker Software. A standard sample mixed by four samples from Coriell Institute, with 20, 29/31, 53, 118 repeats, is used.

Metrics: >95% sensitivity

Terms

By ordering an analysis at Amplexa Genetics, the requester confirms to have obtained the necessary informed consent for the performance of the requested analyses and accepts Amplexa Genetics Terms and Conditions. A hard-copy requisition or an e-mail stating the specific study together with the receipt of a sample is considered an order to conduct the analysis.

From the day of order receipt, the turnaround time is five weeks.