sBACseqFOOD

Objective:

The objective of this project is to advance the analysis of bacterial growth patterns in the food industry by developing a single-cell RNA sequencing (scRNA-seq) platform specifically for bacteria. This will provide detailed insights into the growth patterns of pathogenic bacteria in food production chains, ultimately reducing contamination and spoilage and improving food safety.

Methods:

The methods single cell RNA sequencing has long been used to analyze eukaryotic cells and was recently applied to prokaryotes as well. This method will be used in parallel with bulk RNA sequencing and specifically adapted to pathogenic bacteria from the food industry.

Expected outcomes:

• Robust protocols for RNA stabilization and single-cell isolation of bacteria.
• A functional droplet-based scRNA-seq platform tailored for bacterial analysis.
• Identification of key biomarkers for detecting and preventing bacterial contamination in food products.
• Development of a commercial biomarker kit for the food industry to ensure early detection and prevention of bacterial contamination.

Significance:

This project aims to improve food safety by providing advanced tools to monitor and control bacterial growth in food production. By implementing cutting-edge scRNA-seq technology, the project will enhance the detection of bacterial contamination, reduce food spoilage, and improve public health outcomes. The development of a commercial biomarker kit will offer the food industry a powerful tool to ensure product safety and quality, potentially reducing foodborne illnesses and economic losses due to spoilage.

Keywords: single cell RNA sequencing, bacteria, food waste, listeria, food safety, food contamination, RNA stabilization

In collaboration with Prof. Hanne Ingmer at University of Copenhagen and Matìs in Iceland.

Financed by Eurostars