Construction of bioinformatic infrastructure for transcriptomic analysis to improve induced pluripotent stem cell systems
Objective: The project aims to use computational methods to enhance the maturation of induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) for clinical applications. By identifying the critical differences between immature iPSC-CMs and mature adult cardiomyocytes (CMs), the project seeks to develop bioinformatic solutions that will facilitate the maturation of iPSC-CMs, thereby improving their potential for therapeutic […]
Development of a single cell RNA sequencing platform for bacteria to delineate antibiotic hetero-resistance
Objective: The primary goal of this project is to utilize single-cell RNA sequencing (scRNA-seq) to characterize bacterial populations displaying heterogeneous antibiotic resistance, specifically Staphylococcus aureus isolated from clinical samples. This includes establishing protocols for RNA stabilization, bacterial cell isolation, and sequencing to identify biomarkers for developing diagnostic kits. Methods: The methods single cell RNA sequencing […]